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. 2015 Feb 9;125(3):1228–1242. doi: 10.1172/JCI77656

Figure 8. Knockdown of Ep3a/b variants attenuates hypoxia-induced TGF-β1 activation in PASMCs.

Figure 8

(A–C) Effect of single Ep3a/b/g variant knockdown on mRNA expression of Fn, Col1a1, and Col1a2 in normoxia- or hypoxia-treated PASMCs. (D) Effect of single Ep3a/b/g variant knockdown on active TGF-β1 content in culture medium of normoxia- or hypoxia-treated PASMCs (n = 4). *P < 0.05 versus scramble and #P < 0.05 versus normoxia by 2-way ANOVA with Bonferroni’s post-hoc analysis. (E–G) Western blot analysis of p-SMAD2, FN, and collagen I protein in PASMCs transfected with siRNA specifically designed for Ep3a (E), Ep3b (F), and Ep3g (G), respectively.