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. 2015 Feb 9;125(3):1228–1242. doi: 10.1172/JCI77656

Figure 9. Ep3a/b variants mediate RhoA/ROCK-dependent activation of extracellular MMP-2 in cultured mPASMCs.

Figure 9

(A) Culture MMP-2 activity of PASMCs from Ep3–/– and WT mice. (B) MMP-2 activity in PAs from Ep3–/– and WT mice after normoxia or hypoxia treatment (n = 6). *P < 0.05 versus WT and #P < 0.05 versus normoxia by 2-way ANOVA with Bonferroni’s post-hoc analysis. (C) Culture MMP-2 activity in EP3-deficient PASMCs reexpressing Ep3a, Ep3b, or Ep3g (n = 3). *P < 0.05 versus vector and #P < 0.05 versus normoxia by 2-way ANOVA with Bonferroni’s post-hoc analysis. Effect of Y-27632, wortmannin (Wort), PTX, and H89 on culture MMP-2 activity in EP3-deficient PASMCs with forced expression of Ep3a (D) or Ep3b (E). (F) Western blot analysis of active RhoA in Ep3-deficient PASMCs with forced expression of Ep3a, Ep3b, or Ep3g in the presence of PGE2. pc, pcDNA3.1 vector.