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. 2015 Feb 20;6:517–528. doi: 10.3762/bjnano.6.54

Figure 6.

Figure 6

Cytotoxicity and inflammatory responses of aSNP–plain (100 µg/mL) in combination with alveolar surfactant (Alveofact (0.04 mg/mL), examined on the coculture model of the air-blood barrier A549/ISO-HAS-1. aSNPs were apically (A549) applied simultaneously with Alveofact® in serum-free cell culture medium. A: LDH release was measured after 4 h aSNP incubation (membrane integrity, as xfold of untreated control with a previous normalization to the maximum release (lysis control = 100%)). Cells were then washed and cultured for further 20 h in fresh serum-containing cell culture media (4 h/20 h). B: IL-8 release was measured after 4 h/20 h and depicted as xfold of untreated control (uc). Upper columns: apical compartment, lower columns: basolateral compartment for both LDH and IL-8. Positive control: TNF-α 300 U/mL. Data are depicted as means ± S.D. of 3 independent experiments with n = 3. For statistical analysis two-way ANOVA with Bonferroni´s post test was conducted. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to the untreated control.