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. 2015 Feb 20;6:517–528. doi: 10.3762/bjnano.6.54

Figure 7.

Figure 7

Cellular uptake of aSNP–plain (100 µg/mL, 4 h/20 h) in combination with Alveofact® (0.04 mg/mL) was examined in the coculture of A549 (A–D) with ISO-HAS-1 (E–H). Green signal: E-cadherin counterstaining of A549 (a–d); red signal: uptake of aSNP–plain, which was apically applied to the A549. A/E: control; B/F: A549 treated with Alveofact®; C/G: A549 stimulated with aSNP–plain; D/H: A549 stimulated with aSNP–plain in combination with Alveofact®. E-cadherin staining of A549 shows an inconsistent pattern, although A549 as well as ISO-HAS-1 formed a confluent monolayer. No morphological differences could be observed for A549 and ISO-HAS-1 after stimulation with aSNP–plain, Alveofact® or both. A clear uptake of aSNP–plain was observed with and without Alveofact® treatment in A549, whereas no visual differences could be identified. No aSNP uptake could be detected in ISO-HAS-1, thus negating a transport of aSNPs through stimulated A549 according to this experimental setup. Pictures are taken by a wide field fluorescence microscope (Applied Precision, DeltaVision), nuclei are stained with Hoechst 33433, scale bar 15 µm.