Table 1.
Bacterial strains and plasmids used for biosensor construction.
| Name | Description | References |
|---|---|---|
| BACTERIAL STRAINS | ||
| E. coli JM109 | endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F′ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) | Sigma-Aldrich |
| Pseudomonas sp. ADP | pADP-1, HgR | Mandelbaum et al., 1995 |
| E. coli SM003 | E. coli JM109, pBBluxatzD, pUCatzR, ApR, GmR | This study |
| E. coli SM004 | E. coli JM109, pBBluxatzD, pUCatzABCR, ApR, GmR | This study |
| PLASMIDS | ||
| pBfiluxCDABE | ApR, ptac::luxCDABE, constitutive expression of the Aliivibrio fischeri bioluminescence luxCDABE operon | Charrier et al., 2011 |
| pBfiluxMCS | ApR, pBfiluxCDABE containing MCS and luxCDABE operon | This study |
| pBBR1MCS-5 | GmR, cloning vector rep, lacZ' | NCCB 3437 |
| pBBluxMCS | GmR pBBR1MCS-5 containing MCS and luxCDABE operon | This study |
| pBBluxPatzD | GmR pBBluxMCS containing atzR and PatzDEF promoter | This study |
| pUC19 | ApR, high copy number cloning vector | Invitrogen |
| pUCatzR | ApR pUC19 containing atzR gene | This study |
| pUCatzABC | ApR pUC19 containing atzA, atzB et atzC genes | This study |
| pUCatzABCR | ApR pUCatzR containing atzA, atzB et atzC genes | This study |