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. 2015 Mar 5;6:651–664. doi: 10.3762/bjnano.6.66

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Copyright © 2015, Taylor et al.

This is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)

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Sperm viability parameters after co-incubation of sperm for 2 h at 37 °C with various nanoparticle types and a silver nitrate control. Nanoparticle concentration was 10 µg/mL. (A) Motility assessed with Computer Assissted Sperm Analysis, (B) Membrane integrity assessed with propidium iodide stain and flow cytometer, (C) morphology assessed with phase contrast microscope and evaluation of 200 sperm cells per group per day. Shown are percentage of spermatozoa, which differ compared to the control [values are mean ± SD]. Reproduced with permission from [50]. Copyright 2014 Royal Society of Chemistry.