Table 2.
Biochemical and molecular analysis of rhizosphere and endophytic bacteria isolated from wheat rhizosphere from Rawlakot, AJK.
| Isolates∗ | Biochemical analysis |
Molecular analysis |
|||||
|---|---|---|---|---|---|---|---|
| Catalase activity | Cytochrome oxidase | P-solubilization (mg/mL) | IAA (μg/mL) (with Tryptophan) | IAA (μg/mL) (without Tryptophan) | ARA (μmole C2H4mg protein/h) | Identification based on IGS type/biochemical/16S rRNA gene sequencing | |
| AJK-1 | + | + | 17 | 59.19 | 38.34 | - | ARS1 |
| AJK-2 | + | - | 16 | 10.3 | 5.9 | 1.64 | ARS2 |
| AJK-3 | + | + | 16 | 72.32 | 62.45 | 1.78 | ARS3 Stenotrophomonas rhizophila |
| AJK-4 | + | + | 2 | 1.88 | 0 | 2.25 | ARS4 Bacillus sp. |
| AJK-5 | + | - | 2 | 0 | 0 | 1.44 | ARS2 |
| AJK-6 | + | + | 2 | 0.27 | 0 | 7.89 | ARS3 |
| AJK-7 | + | + | 8 | 7.56 | 0.12 | 1.53 | ARS2 Acetobactor pasteurianus |
| AJK-8 | + | + | 19 | 0.56 | 0 | - | ARS4 Bacillus sp. |
| AJK-9 | + | - | 4 | 77.98 | 67.54 | 2.78 | ARS3 Stenotrophomonas |
∗All isolates produced substantial amount of gum on LB agar plates but non-solubilized Zinc oxide or Zinc sulfate in vitro. ARA, Acetylene reduction assay; IAA, Indole acetic acid was tested with and without the addition of precursor; Data are the mean values of three replicates each and all experiments were repeated twice for confirmation of results. The symbol, + representing the positive reaction or the presence of the traits while the symbol, - shows the negative reaction or absence of the component/trait.