Figure 1.

Lectin histochemistry in NA-injected rat brains immediately after the injection. (A,B) The site of injection is indicated with a red arrow in (A). Note a LFA-negative and PNA-positive halo around the injected lateral ventricle (i) and the third ventricle (IIIv) (double-headed arrow) but not the contralateral ventricle (cl). (C–F) Details of the zones squared in (A,B) showing the negative or positive halo (double-headed arrows). The squared areas are shown in detail in the following pictures. (G–J) The surface of the ependymal cells was LFA-negative (G) and PNA-positive (I) in the injected ventricle, whereas it was LFA-positive (H) and almost PNA-negative (J) in the contralateral ventricle. cc, corpus callosum; cl, contralateral ventricle; i, injected lateral ventricle; s, striatum; sf, septum-fimbria; IIIv, third ventricle. Sections in (A,C,D,G,H) were counterstained with hematoxilin–eosin.