Table 1.
Statistics of the EST raw reads and assembly.
A. elongata | Collozoum sp. | S. streptacantha | A. scolymantha | Total | ||
---|---|---|---|---|---|---|
Total sequenced | Raw reads | 195,890 | 214,475 | 220,239 | 195,070 | 825,674 |
Cleaned reads | 138,867 | 121,079 | 120,924 | 126,494 | 507,364 | |
BP | 5.0 × 107 | 4.2 × 107 | 4.2 × 107 | 4.1 × 107 | 1.7 × 108 | |
Average length | 362 | 337 | 348 | 369 | ||
Median length | 365 | 324 | 338 | 378 | ||
After assembly | Unigenes | 14,210 | 42,784 | 25,590 | 42,251 | 124,835 |
BP | 6.5 × 106 | 1.6 × 107 | 1.0 × 107 | 1.7 × 107 | 4.9 × 107 | |
GC % | 46.3 | 44.0 | 50.9 | 40.6 | ||
Coverage | 8.6 | 3.3 | 5.2 | 3.2 | ||
Contigs | No | 3252 | 4888 | 3229 | 3243 | 14,612 |
Assembly size (BP) | 2.8 × 106 | 3.4 × 106 | 2.8 × 106 | 2.9 × 106 | 1.2 × 107 | |
%BP assembled | 42.4 | 21.6 | 26.9 | 16.9 | ||
Average length | 858 | 699 | 883 | 893 | ||
Median length | 663 | 544 | 606 | 637 | ||
Annotations | Blastn | 410 | 153 | 1486 | 409 | 2458 |
Blastx | 13,800 | 42,631 | 24,104 | 41,842 | 122,377 | |
Unigenes annotated to GO | 2798 | 4839 | 4662 | 6938 | 19,237 | |
GO terms | 2002 | 3027 | 3239 | 4318 | 6077 | |
Unigenes annotated to KEGG | 1303 | 1665 | 2264 | 2047 | 7279 | |
KEGG pathways | 98 | 101 | 105 | 111 | 123 |