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. 2014 Apr 2;1:14011. doi: 10.1038/mtm.2014.11

Figure 2.

Figure 2

Generation of CHM1 iPSCs. (a) Fibroblasts of patient CHM1 in culture. Bar = 100 µm. (b) Lentiviral transduction and doxycycline induction results in the expression of the four reprogramming factors, as determined by quantitative polymerase chain reaction analysis. (c) Successful reprogramming results in the detection of an iPSC colony composed of tightly packed cells with a clear border separating them from the underlying fibroblasts. Bar = 200 µm. (d) The deletion in CHM1 results in the amplification of exons 7 and 9 in patient fibroblasts (Fib) and iPSCs, but in the absence of exon 8, as opposed to the situation in wild-type (WT) DNA; − indicates negative control. (e) At the complementary DNA (cDNA) level, the CHM1 transcript is 226 bp smaller, corresponding to the deletion of exon 8. (f) No large chromosomal anomalies in CHM1 iPSCs were detected by karyotype analysis. iPSC, induced pluripotent stem cell.