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Plasmid construct. Schematic representation of the circular enhanced expression vector (EEV) plasmid constructed for this study. The circular pEEV construct incorporates a CMV IE/T7 promoter, an Semliki Forest virus (SFV) replicase (nonstructural proteins 1–4), nuclear localization sequence, the entire SFV capsid gene, which functions as a self-cleaving translation enhancer, an Ori colE1 (Ori), 26S subgenomic promoter, capsid (CAP) gene and an ampicillin resistance cassette (AmpR).
