Table 1. Clinical examination of NSG mice treated with Mreg_UKR.
| Vehicle-only | 106 M regs | 107 M regs | |
|---|---|---|---|
| Behavioral | |||
| Spontaneous activity | |||
| Sleep | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Body position | 5.2 P0 | 5.6 P0 | 5.6 P0 |
| Locomotor activity | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.8 ± 1.1 |
| Bizarre behavior | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Motor-affective response | |||
| Alley progression (cm) | 35.5 ± 14.7 | 43.9 ± 20.5 | 45.0 ± 22.3 |
| Transfer arousal | 4.0 ± 0.0 | 4.0 ± 0.0 | 3.2 ± 1.8 |
| Touch-escape | 3.6 ± 0.9 | 4.0 ± 0.0 | 4.4 ± 0.9 |
| Positional struggle | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.0 ± 0.0 |
| Grasp irritability | 0.8 ± 0.4 | 0.8 ± 0.4 | 1.0 ± 0.7 |
| Provoked biting | 3.2 ± 1.8 | 2.4 ± 2.2 | 2.0 ± 2.0 |
| Provoked freezing | 1.0 ± 0.0 | 0.8 ± 0.4 | 1.0 ± 0.0 |
| Finger approach | 4.8 ± 1.1 | 5.2 ± 1.1 | 5.2 ± 1.1 |
| Positional passivity | 3.2 ± 1.1 | 3.6 ± 1.7 | 2.4 ± 0.9 |
| Vocalization (events) | 0.0 ± 0.0 | 0.6 ± 0.9 | 0.6 ± 0.9 |
| Urination (events) | 0.2 ± 0.4 | 0.2 ± 0.4 | 0.2 ± 0.4 |
| Defecation (events) | 2.4 ± 2.1 | 1.0 ± 1.7 | 4.0 ± 1.9 |
| Sensory-motor response | |||
| Visual placing | 6.0 ± 0.0 | 6.0 ± 0.0 | 5.6 ± 0.9 |
| Tail-pinch | 2.6 ± 2.3 | 1.0 ± 0.0 | 2.4 ± 1.5 |
| Toe-pinch reflex | 5.6 ± 0.9 | 5.6 ± 0.9 | 6.0 ± 0.0 |
| Corneal reflex | 5.2 ± 1.1 | 4.8 ± 1.1 | 5.6 ± 0.9 |
| Pinna reflex | 2.4 ± 0.9 | 2.4 ± 0.9 | 3.6 ± 0.9 |
| Startle | 2.8 ± 1.1 | 5.2 ± 1.8 | 4.0 ± 1.4 |
| Neurological | |||
| Posture | |||
| Pelvic elevation | 4.0 ± 0.0 | 4.4 ± 0.9 | 4.4 ± 0.9 |
| Tail elevation | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.0 ± 0.0 |
| Limb rotation | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.0 ± 0.0 |
| Muscle tone | |||
| Body tone | 6.0 ± 0.0 | 6.0 ± 0.0 | 6.0 ± 0.0 |
| Abdominal tone | 4.0 ± 0.0 | 4.4 ± 0.9 | 5.2 ± 1.1 |
| Limb tone | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.0 ± 0.0 |
| Grip strength | 6.0 ± 0.0 | 5.6 ± 0.9 | 4.8 ± 01.8 |
| Wire maneuver | 0.4 ± 0.9 | 0.8 ± 1.8 | 0.4 ± 0.9 |
| Equilibrium and gait | |||
| Righting reflex | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Ataxic gait | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Hypotonic gait | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Other gait impairment | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Total gait incapacity | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| CNS excitation | |||
| Tremors | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Twitches | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Convulsions | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Autonomic | |||
| Eyes | |||
| Palpebral closure | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Exopthalmos | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Secretion and excretion | |||
| Lacrimation | 1.2 ± 2.7 | 0.0 ± 0.0 | 0.2 ± 0.4 |
| Salivation | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Diarrhea | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| General | |||
| Hypothermia | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Skin color | 4.0 ± 0.0 | 4.0 ± 0.0 | 4.0 ± 0.0 |
| Resp. rate | 6.0 ± 0.0 | 6.0 ± 0.0 | 6.0 ± 0.0 |
| Toxicity | |||
| Acute death (events) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
NMRI-nude mice were allocated to three groups of five animals. Mice received injections of either 106 or 107 viable human M regs resuspended in Ringer’s lactate solution plus 5% human serum albumin and 60 U heparin, or were given a vehicle-only injection. Seven days after Mreg_UKR administration, recipient mice underwent clinical examination to identify possible signs of neurological impairment. No significant differences were found. Values represent mean ± SD.