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. 2014 Jul 2;1:14027. doi: 10.1038/mtm.2014.27

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Copyright © 2014 The American Society of Gene & Cell Therapy

This work is licensed. under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Intracellular IFN-γ staining of FMP-specific CD8⁺ T cells in mice immunized with Ad-SCT-H74L. HHD mice (five to nine per group) were immunized with Ad-WT, Ad-FMP, Ad-SCT-HHD or Ad-SCT-H74L at various inoculation doses. After 1 week, splenic lymphocytes were prepared, and stimulated with (+) or without (−) FMP_58–66 for 5 hours. Cells were then stained for their surface expression of CD8 and their intracellular expression of IFN-γ. The data indicate the percentages of INF-γ-producing cells within CD8⁺ T cells. (a) Each symbol represents an individual mouse. Horizontal bars represent the mean. *P < 0.05; NS, not significant, One-way analysis of variance. (b) Data are representative in mice immunized with Ad-WT or Ad-SCT-H74L. The numbers shown indicate the percentages of INF-γ-secreting cells within CD8⁺ T cells.