Figure 7.
Biodistribution of AAV2/8-LSPhGAApA vector in mouse tissues. Tissues were collected from mice administered AAV2/8-LSPhGAApA vector at 1.6 × 1013 vp/kg via tail vein injection at 2, 8, and 16 weeks postdosing. The vector genome copies were analyzed by quantitative polymerase chain reaction (qPCR) as copies/µg genomic DNA. The lowest limit of quantitation of the qPCR assay was 38 copies/µg genomic DNA. The data were presented as geometric mean (n = 5). The positive error bar represents the upper limit of 95% confidence interval, and the negative error bar the lower limit of 95% confidence interval. M tissue was from male mice; F tissue was from female mice. Note that only data from vector-dosed groups (group 10 for 2 weeks, group 12 for 8 weeks, and group 14 for 16 weeks) were presented, and vector genome copy numbers in the vehicle control animal tissues from group 9, 11, and 13 were all below the lower limit of quantification of the qPCR assay (data not shown).