Figure 5.

GSK enhances Ca²⁺ spark frequency in TRPC3-dependent manner. Confocal line scan images were recorded along the longitudinal axis of myocytes loaded with fluo-4 as described previously³⁶ and analysed using custom-made algorithms coded in IDL.^36,37 Left: time plots of Ca²⁺-sensitive fluorescence line scans recorded from WT cardiomyocytes (n = 14; N = 3), TRPC3-TG (n = 19; N = 7), WT + GSK (n = 19; N = 3), and TRPC3-TG + GSK (n = 19; N = 5); Right: mean calcium spark frequency (± SEM). Intracellular Ca²⁺ fluxes were at equilibrium and measurement was performed during a prolonged diastole. Sharp (^#) indicates significant difference (P < 0.05) in comparison to WT, * indicates significance (P < 0.05) in comparison to untreated TRPC3-TG cells. Statistical significance analysed by two-way Anova followed by Tukey's post hoc tests.