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. 2015 Feb 17;106(1):55–66. doi: 10.1093/cvr/cvv040

Figure 7.

Figure 7

Robo activates CBFRE-luciferase activity. (A) Robo and Notch are both membrane-bound receptors with intracellular signalling domains. Cleavage and binding of the intracellular domain of Notch to Cbf1 activates the CBFRE-luciferase reporter construct. (B and C) CBFRE-luciferase activation in the presence of the indicated transfected genes in COS7 cells. (B) P = 0.65, P = 0.045, P = 0.01 compared with NICD1 alone, respectively. Robo1 and 2 with NICD1 compared with Robo1 with NICD1 P = 0.007, to Robo2 with NICD1 P = 0.041, n = 5. (C) P = 0.004, P = 0.004, P = 0.14 compared with NICD1 alone, respectively, n = 5; one-way ANOVA with Bonferroni's multiple comparison test. (D) Scheme showing the similarity of the expression patterns between the Slit-Robo and Notch-Hey genes, with an overview of the observed defects. Luc, luciferase. *P < 0.05, **P < 0.01. For other abbreviations, see the legend of Figure 1.