Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 16;208(6):745–759. doi: 10.1083/jcb.201409056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ghalei et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — The essential function of human CK1δ is in ribosome maturation. (A) Cell proliferation (as determined by doubling times) of control MDA-MB-231 cells, or of MDA-MB-231 cells where hLtv1 was silenced by Dox-directed induction of hLtv1 shRNA for 3 d, before addition of 30 nM SR3029 or vehicle for another 3 d. (B) Western blot analyses established ∼80% depletion of Ltv1 after 3 d of Ltv1 knockdown. (C) Proliferation of MDA-MB-231 cells engineered to inducibly overexpress wild-type hLtv1, hLtv1-S/D, or hLtv1-S/A after Dox treatment for 3 d. (D) Annexin V/DAPI staining combined with FACS analysis of control MDA-MB-231 cells and those depleted of hLtv1 in the presence or absence of 100 nM SR-3029. All measurements in A, C, and D were done in triplicate, and error bars show the standard deviation of these data.