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. 2015 Jan;6(1-2):9–18. doi: 10.18632/genesandcancer.48

Figure 4. Histological examination of brain sections from RCASBP(A) BRAF-KD and CRE injected mice.

Figure 4

A: Representative low-power images of brain sections from tumor-bearing mice are shown. H&E: sections stained with hematoxylin and eosin. IHC for synaptophysin demonstrated that none of the tumors were neuronal in origin. Tumor A is representative of tumors that expresses the astrocyte maker GFAP while tumor B is an example that was negative for GFAP expression. Cells in tumor C possessed spindle cell morphology, while tumor B was more epithelioid, and tumor A was mixed as well as containing a population of large non-dividing giant cells. Scale bar represents 1mm. B: High power histological examination of a GFAP expressing tumor. IHC for synaptophysin demonstrates that the tumors are not neuronal and expresses the astrocyte maker GFAP. BRAF-KD expression was detected by IHC for the HA epitope tag on virally delivered BRAF. IHC for P-Erk demonstrates that the MAPK pathway is active in the tumor but not adjacent normal brain. IHC sections were counterstained with hematoxylin. Scale bar represents 200μm.