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. 2015 Jan;6(1-2):30–37. doi: 10.18632/genesandcancer.47

Figure 3. Time-dependent changes of p300 autoacetylation in the presence and absence of E1A 1-80.

Figure 3

A. p300 autoacetylation at different times of reaction. In vitro acetylation reactions were performed with p300, VP16 (Gal4-VP16), and assembled chromatin, in the presence or absence of E1A 1-80 (264 nM). Assays were performed at 320C for 5, 10 or 20 minutes as indicated. Western blots were performed with antibodies as indicated. Acetylated p300 (Ac-p300) was detected with the Ac-Lys antibody (top panel).

B. Quantitation of autoacetylated p300. Western blot signals from A were quantitated by chemiluminescence directly on a Bio-Rad scanner. H3K18 acetylation reached near maximum at 5 min of reaction and inhibition by E1A 1-80 was greater than 97% for all three time points (not shown).