FIGURE 6.

Peroxidase activity in cells treated with TFF3. Peroxidase activity was measured according to the 2-nitrobenzoic acid-thiocyanate (NBS-SCN) assay as previously described [15]. HT-29 cells were grown in 6-well plates to 50% confluence and serum-starved for 24 h. Cells were then treated for 48 h with 10 μg/ml trefoil. In some experiments HT-29 cells were preincubated for 24 h with LPS (1 μg/ml) before treating with trefoil for 48 h. Afterwards, culture supernatants of HT-29 were collected, centrifuged at 1000g for 15 min at 4°C, and peroxidase activity was measured.