Fig. 2.

Methylation compared to protein expression for Apaf1, Bcl2 and p53. Interpolation plots showing methylation versus protein expression for Apaf1, Bcl2 and p53. Protein expression was scored as percentage of tumor cells showing negative, weak, moderate or strong IHC staining. The histoscore was calculated as follows: histoscore = (0 * percentage negative) + (1 * percentage weak) + (2 * percentage moderate) + (3 * percentage strong). R² values indicate the degree of correlation between methylation and protein expression for each of the markers