The effects of Lipofectamine2000, US and US plus MBs on retinal cell viability assessed by trypan blue exclusion test at 12 hour after intravitreal injection. US, ultrasound exposure (1MHz, 2W/cm2, 50%, 100Hz, 300 seconds); MBs, SonoVue® microbubble contrast agents (2μL); L, Lipofectamine2000 (0.2 nmol).