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. 2015 Mar 3;49(1):41–49. doi: 10.2478/raon-2014-0045

FIGURE 2.

FIGURE 2.

Recognition of HER-2 by Herceptin® following mHT. BT-474 cells were incubated with 10 μg/mL native or heated Herceptin®, or matched human isotype control IgG1. Subsequently, the cells were incubated with FITC-conjugated anti-human IgG1-Fc antibody and then analyzed by confocal microscopy (A) and flow cytometry (B). Representative images of BT-474 cells and dot-plots of each experimental condition are shown. Data expressed as mean ± SD was calculated from three independent experiments. Statistical analysis was performed using the non-parametric Mann-Whitney test. Significance was defined as p < 0.05 (NS, non-significant).