Figure 6.

Western blot analysis of the mTOR mediated insulin-stimulated pathway. (A) Overview of the growth-regulating mTOR signaling pathway. (B) Western blot analyses of protein extracted from skeletal muscle showing blots of phosphorylated (upper panel) and not phosphorylated (lower panel) up- and down-stream regulators of the mTOR pathway in liraglutide- (left side of the blot) and placebo-treated (right side of the blot) GIPR^dn transgenic pigs. For elF4E not phosphorylated molecule (upper panel) is referred to tubulin (lower panel). (C) Quantitative analysis of Western blot signals, results are expressed as ratio of phosphorylated to not phosphorylated regulator and for eIF4E as absolute fold expression, related to the mean value of the placebo group, respectively, and presented as box plots with median, n = number of animals investigated, black plots = liraglutide-treated animals, white plots = placebo-treated animals, * = p < 0.05.