TABLE 1.
Primer Sequences (5′–3′) and Optimized Polymerase Chain Reaction Conditions
| Region | Forward 5′–3′ | Reverse 5′–3′ | [Mg] | °C |
|---|---|---|---|---|
| Promoter 1 | CCACGTTTCTTTCGCCCCCTC | ACTGCGGAGGAGGCAGCTTG | 3 | 60 |
| Promoter 2 | CGCCCGCCCCTGATTTCCTC | TTCTGCTGACAGCGGCTCCAT | 3 | 60 |
| Promoter 3 | GAGCAGCCGCGCTCGGAGAA | GAAGTCGATGGCTATGTTGG | 3 | 60 |
| Exon 1 | AGTCCCCTCGCGGCAGCAGA | GCTGGAAAAGTCACCCTTGA | 3 | 60 |
| Exon 2 | ACCCTATAAGCTACTTAGTG | AGAAACATTTGATAATTAGTG | 3 | 50 |
| Exon 3 | TGACCCACTCTGGGTAGAGG | CCTGCCATTAAGCTGTACACAC | 3 | 60 |
| Exon 4 | TGCAGTCCTTCACTCCACTG | CAGTTACACACGCCAGAAGG | 2 | 60 |
| Exon 5 | CCCCTGTGCTTCTGTCAGTC | CTGTCTTTCCCTGCAGACATC | 2 | 60 |
| Exon 6 | GGCTTGTGATTAACATACGAAGG | CACCGAACGAGATCTTTATGG | 3 | 60 |
| Exon 7 | CGTGCTTCGTCACCTACTGT | CCCCAACGTCACATTAACCT | 2 | 60 |
| Exon 8 | GCCCGAGAGACACTCAACAT | TGCCCCTTTACAAAAACCAA | 2 | 60 |
| Exon 9 | AGCAAGCAGGTGGCAGATCTG | AGTGAAATTTTACATTTGTG | 3 | 50 |
| Exon 10 | ACCCTGGCAGGAAGATGAG | CCCAAACTCCCTGACAACAT | 2 | 60 |
| Exon 11 | GAAGCCAGCAGATGGAGAAC | ACCATCCAACCTGGTCAGTG | 3 | 60 |
| Exon 12.1 | CAACCAAACTGCAAGGAACA | GCCGAAGTGTCATCCTGACT | 2 | 60 |
| Exon 12.2 | AGAATGAATAAGGCACGGGACG | ACAACGTCAACCGTGAGGCAG | 2 | 60 |