Figure 4.

In vivo endotoxin triggers metabolic perturbations in human leukocytes. Blood samples were obtained at the times indicated from subjects described in Figure 1 who were challenged with endotoxin at either (A) 1 ng/kg or (B) 0.1 ng/kg. Leukocyte lysates containing equal protein amounts were subjected to Western blotting and ATP analyses. (C) Schematic contrasting the in vivo endotoxin response at doses of 1 ng/kg and 0.1 ng/kg. Both low- and high-dose endotoxin initiate metabolic perturbations in human leukocytes. In contrast, only high-dose endotoxin triggers robust inflammatory responses in all subjects.