. Author manuscript; available in PMC: 2015 Mar 17.

Published in final edited form as: Nat Commun. 2013;4:2924. doi: 10.1038/ncomms3924

Table 1.

23GFP⁺ HE undergoes dynamic functional changes early in development.

		No. of wells with haematopoietic and/or endothelial progeny/no. wells seeded with a single cell (frequency)
Cell type	Stage	Haematopoietic and endothelial progeny	Haematopoietic progeny	Endothelial progeny
23GFP⁺ HE	E8.5	0/805	0/805	55/805 (1:15)
	E9.5	0/540	1/540 (1:540)	8/540 (1:68)
	E10.5	0/539	11/539 (1:49)	5/539 (1:108)
23GFP^– EC	E8.5	0/288	0/288	27/288 (1:11)
	E9.5	0/234	0/234	13/234 (1:18)
	E10.5	0/192	0/192	7/192 (1:27)

EC, endothelial cells, GFP, green fluorescent protein; HE, haemogenic endothelium; sp, somite pair.

Haemogenic and endothelial tubule forming potential of individual 23GFP⁺ HE cells, as determined by clonal OP9 cocultures. Data are the total of 2-5 independent experiments performed on pooled tissues of E8.5 PAS + YS (4-10 sp), E9.25-9.5 PAS + VU (17-27 sp), E10.5 AGM + VU (32-37 sp), apart from E10.5 23GFP cultures (1 experiment). Similar to the bulk cultures, 23GFP EC were unable to generate haematopoietic progeny.