Table 1.
No. of wells with haematopoietic and/or endothelial progeny/no. wells seeded with a single cell (frequency) |
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Cell type | Stage | Haematopoietic and endothelial progeny | Haematopoietic progeny | Endothelial progeny |
23GFP+ HE | E8.5 | 0/805 | 0/805 | 55/805 (1:15) |
E9.5 | 0/540 | 1/540 (1:540) | 8/540 (1:68) | |
E10.5 | 0/539 | 11/539 (1:49) | 5/539 (1:108) | |
23GFP– EC | E8.5 | 0/288 | 0/288 | 27/288 (1:11) |
E9.5 | 0/234 | 0/234 | 13/234 (1:18) | |
E10.5 | 0/192 | 0/192 | 7/192 (1:27) |
EC, endothelial cells, GFP, green fluorescent protein; HE, haemogenic endothelium; sp, somite pair.
Haemogenic and endothelial tubule forming potential of individual 23GFP+ HE cells, as determined by clonal OP9 cocultures. Data are the total of 2-5 independent experiments performed on pooled tissues of E8.5 PAS + YS (4-10 sp), E9.25-9.5 PAS + VU (17-27 sp), E10.5 AGM + VU (32-37 sp), apart from E10.5 23GFP cultures (1 experiment). Similar to the bulk cultures, 23GFP EC were unable to generate haematopoietic progeny.