Skip to main content
. Author manuscript; available in PMC: 2015 Mar 17.
Published in final edited form as: Gut. 2014 Jan 16;63(10):1658–1667. doi: 10.1136/gutjnl-2013-305281

Figure 3.

Figure 3

Baseline matrix metalloprotease (MMP) hyperactivity of polycystic human cholangiocytes is autocrine/paracrine stimulated by interleukin (IL)-6 and/or IL-8. (A) mRNA levels of IL-6 and IL-8 are overexpressed in polycystic versus normal human cholangiocytes in culture. (B) Extracellular secretion of both IL-6 and IL-8 proteins is increased in polycystic versus normal human cholangiocytes in culture. (C) The presence of antibodies against IL-6 receptor (IL-6R) and/or IL-8 receptor (IL-8R) in the culture medium inhibited baseline MMP hyperactivity of polycystic human cholangiocytes compared with cells incubated with a negative control antibody. (D) In contrast, the presence of antibodies against IL-6R and/or IL-8R did not affect baseline MMP activity of normal human cholangiocytes in culture. n, number of wells analysed in each.