Fig 6. Reversibility assay of HIV-1 PR of 5 μM concentration (expressed as dimer).

For every enzyme sample three CSM values were determined: at 10 MPa before pressurizing, at the selected target pressure from the interval of 100 to 350 MPa after 50 min incubation and again at 10 MPa after releasing the pressure. The experiment indicates good reversibility of CSM up to 300 MPa, above this value the reversibility is perturbed by protein aggregation.