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. 2014 Nov 13;2:20. doi: 10.1186/2050-7771-2-20

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© Orro et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Measurements of IL-1 α, IL-1RA, CXCL-1/2 and hBD-1 from healthy skin using TAP and from skin lavage techniques. TAP measurements of IL-1α, IL-1RA, CXCL-1/2 and hBD-1 from healthy skin were analyzed and compared with three different methods using skin lavage. See text for details. Signal intensities for the different methods were compared to signals obtained using fixed concentrations of recombinant IL-1α, IL-1RA, CXCL-1/2 and hBD-1 captured in solution. In the graphs, it is clearly visible that TAP is the most sensitive method for the detection of IL-1α (panel A), IL-1RA (panel B) and hBD-1 (panel D) on skin. CXCL-1/2 (panel C) could not be detected by any of the methods. Each data point in the graphs represents the results of two spots analyzed on 20 TAP’s (N = 10). Y-axis: Apparent concentration of IL-1α, IL-1RA, CXCL-1/2 or hBD-1 on skin in ng/ml. X-axis: Incubation time on skin in minutes. Error bars on graph present standard deviation of analyzed data points (N = 10).