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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Bull Math Biol. 2014 Sep 4;77(5):796–816. doi: 10.1007/s11538-014-0009-9

Figure 7. Unreplicated DNA raises the cyclin threshold for MPF activation in frog egg extracts.

Figure 7

Images from Sha et al. (2003); used by permission. (A) Interphase arrested extracts are supplemented with 0 or 40 nM non-degradable cyclin B at t = 0 and checked for entry into mitosis at t = 40 and 140 min. The cyclin threshold for activation of MPF is less than 40 nM, as in Fig. 5 and 6. (B) Same as panel A, except that aphidicolin (an inhibitor of DNA synthesis) is added to the extract at t = 40 min. In the presence of unreplicated DNA, the cyclin threshold for MPF activation is raised to ~90 nM.