Figure 6.

MG132, a proteasome inhibitor, abolished em08red-mediated ErbB2 protein downregulation.

Notes: In the cotreatment group, HSC3 cells were preincubated with MG132 (10 μM) for one hour, and em08red (10 μM) was then added to each treatment group for a further 16 hours of incubation. Cells were harvested at the indicated times and the total cell lysates were subjected to Western blot analysis. Antibodies specific for total ErbB2 and actin were used to detect protein expression after treatment. Actin expression served as the loading control.