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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 16;1844(12):2135–2144. doi: 10.1016/j.bbapap.2014.09.009

Table 1.

Methylation activity of SD_1168 upon various substrates. Unless otherwise noted, reactions were incubated anaerobically for ~18 h prior to NMR analysis. NR = No reaction.

Enzyme
preparation		 Reductant Organophosphorus
substrate		 Cobalamin
substrate		 SAM
present(+)/
absent(−)		 %
Turnover		 [Product]
(μM)		 Figure
− Ti(III)
citrate		 2-HEP CH3Cbl + NR NR −
SD_1168 Na2SO4 2-HEP CH3Cbl + NR NR S2
SD_1168 Ti(III)
citrate		 2-HEP CH3Cbl + NR NR −
− Ti(III)
citrate		 NAcDMPT CH3Cbl + NR NR S4A
SD_1168 Na2SO4 NAcDMPT CH3Cbl + 0.3% 18 S3
SD_1168 Ti(III)
citrate		 NAcDMPT CH3Cbl + 3.7%
(5 min)		 220 Not
shown
SD_1168 Ti(III)
citrate		 NAcDMPT CH3Cbl + 4.8%
(2 h)		 290 Not
shown
SD_1168 Ti(III)
citrate		 NAcDMPT CH3Cbl + 7.4% 400 5A
SD_1168 − NAcDMPT CH3Cbl + NR NR S4B
SD_1168 Ti(III)
citrate		 NAcDMPT − + NR NR S4C
SD_1168
(refolded
without
exogenous
SAM)		 Ti(III)
citrate		 NAcDMPT CH3Cbl − 8.4% 460 S4D
SD_1168(−
SAM)		 Ti(III)
citrate		 NAcDMPT CH3Cbl − NR NR S6
SD_1168 Ti(III)
citrate		 NAcDMPT HOCbl + 8.5% 460 5C
SD_1168 Ti(III)
citrate		 NAcDMPT [methyl-
13C]Cbl		 + 11.0% 1100 5D
SD_1168 Ti(III)
citrate		 DMPT CH3Cbl + 3.5% 190 5B