Fig 3. Heteromeric ASIC currents are insensitive to PI(4)P and PI(4,5)P₂.

(A) Current traces from ASIC1a/2a, ASIC1a/3, and ASIC2a/3 heteromeric channels evoked by extracellular acidification in cells expressing LDR and PJ. Rapamycin (1 μM) was bath-applied for 60 s, and then normal extracellular solution was perfused for 10 s right before the second pulse to minimize possible side effects of rapamycin. Dashed line indicates the zero current level. (B) Relative current density measured for the currents of ASIC1a/2a and ASIC1a/3 in (A) (n = 3, respectively). Current density of each pulse was divided by that of the first pulse. There is no statistical significance with two-way ANOVA followed by Bonferroni post-hoc test. (C) Relative current density measured for the transient and sustained currents of ASIC2a/3 in (A) (n = 3 for PJ-Dead; n = 5 for PJ). There is no statistical significance with two-way ANOVA followed by Bonferroni post-hoc test. Data are mean ± SEM.