Fig. 2.

Inhibition of nitrite production and iNOS protein expression by propofol in LPS-stimulated canine PBMCs. (A) Propofol inhibits NO production in LPS-stimulated canine PBMCs. (B) Propofol inhibits iNOS protein expression in LPS-stimulated canine PBMCs. Canine PBMCs were pretreated with different doses of propofol (0, 25 or 50 µM) for 6 hr and then stimulated with LPS (100 ng/ml) for 24 hr. The concentration of NO in the culture medium was determined by using a Griess assay. Cell lysates were extracted, and the protein levels of iNOS were detected by Western blotting. β-actin was used as a loading control. Densitometry was normalized to β-actin and graphed as the mean ± S.D. *indicates P<0.05 (compared with LPS stimulation alone). Similar results were obtained from three independent experiments.