Fig. 4.

Propofol inhibits the nuclear translocation of the NF-κB p65 protein in LPS-stimulated canine PBMCs. Canine PBMCs were pretreated with different doses of propofol (0, 25 or 50 µM) for 6 hr and then stimulated with LPS (100 ng/ml) for 1 hr. The NF-κB p65 protein was analyzed by Western blotting. Histone H3 was used as a loading control. Densitometry was normalized to Histone H3 and graphed as the mean ± S.D. *indicates P<0.05 (compared with LPS stimulation alone). Similar results were obtained from three independent experiments.