Table 1.
Statistical summary of sequencing and assembly results
| 0 h | 6 h | 12 h | 24 h | 72 h | |
|---|---|---|---|---|---|
| Total Raw Reads | 60,099,376 | 59,434,584 | 59,981,694 | 58,988,346 | 59,451,888 |
| Total Clean Reads | 53,035,664 | 51,344,528 | 53,343,062 | 52,930,276 | 54,646,950 |
| Total Clean Nucleotides (nt) | 4,773,209,760 | 4,621,007,520 | 4,800,875,580 | 4,763,724,840 | 4,918,225,500 |
| Q20 Percentage | 97.68% | 97.77% | 97.70% | 97.74% | 97.66% |
| N Percentage | 0.00% | 0.00% | 0.00% | 0.00% | 0.00% |
| GC Percentage | 43.90% | 44.20% | 43.79% | 43.74% | 44.08% |
| Total Contigs | 75,823 | 76,045 | 69,207 | 73,309 | 67,563 |
| Total Length (nt) | 32,435,990 | 30,098,496 | 29,746,317 | 31,507,438 | 30,173,681 |
| Mean Length (nt) | 428 | 396 | 430 | 430 | 447 |
| N50 | 913 | 785 | 967 | 918 | 991 |
| Total Unigenes | 58,699 | 60,883 | 51,573 | 57,557 | 53,264 |
| Total Length (nt) | 33,330,719 | 29,510,487 | 29,170,625 | 31,846,285 | 29,670,894 |
| Mean Length (nt) | 568 | 485 | 566 | 553 | 557 |
| N50 | 850 | 672 | 826 | 820 | 800 |
| All Unigenes | Total Number: 50,267; Total Length (nt): 43,714,924; Mean Length (nt): 870; N50: 1441 | ||||
Note: The Q20 percentage is the proportion of nucleotides with a quality value > 20. The N percentage is the proportion of unknown nucleotides in clean reads. The GC percentage is the proportion of guanidine and cytosine nucleotides among total nucleotides. N50 is 50% of the assembled bases that were incorporated into sequences with a length of N50 or longer.