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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Fertil Steril. 2015 Jan 14;103(3):845–852. doi: 10.1016/j.fertnstert.2014.12.099

Figure 1. ELISA quantification of TGF-β3 in ESC-conditioned and Leiomyoma-Conditioned media.

Figure 1

TGF-β3 protein concentration was quantified by ELISA in conditioned media from primary ESC and leiomyoma cells grown at 60–70% confluence and cultured for 24 hours. Mean TGF-β3 protein concentration was significantly higher in leiomyoma-conditioned media compared to ESC-conditioned media (52 ng/ml vs. 9.2 ng/ml, p<0.05). TGF-β3 protein was present in low concentration (3.2 ng/ml) in standard media (DMEM containing 1% penicillin, 1% streptomycin, 1% Amphotericin B, and 10% fetal bovine serum).