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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Fertil Steril. 2015 Jan 14;103(3):845–852. doi: 10.1016/j.fertnstert.2014.12.099

Figure 3. BMP-2 stimulated HOXA10 and LIF expression in LCM treated ESC: Effect of TGF-β Antibody.

Figure 3

Figure 3

BMP-2 responsiveness was assessed in LCM exposed ESC by treatment with rhBMP-2. HOXA10 and LIF expression were quantified by qRT-PCR after 24 hours of rhBMP-2 treatment. A: BMP-2 stimulated HOXA10 expression. BMP-2 stimulated HOXA10 expression was unchanged in MCM exposed ESC, compared to non-exposed control ESC (1.07 fold, NS). BMP-2 stimulated HOXA10 expression was repressed in ESC exposed to LCM, compared to non LCM exposed control ESC (0.63 fold, p<0.05). Incubation of LCM with TGF-β neutralizing antibody, prior to ESC exposure, prevented repression of BMP-2 stimulated HOXA10 expression. B: BMP-2 stimulated LIF expression. BMP-2 stimulated LIF expression was unchanged in MCM exposed ESC, compared to non-exposed control ESC (0.94 fold, NS). BMP-2 stimulated LIF expression was repressed in LCM exposed ESC, compared to non LCM exposed control ESC (0.29 fold, p<0.05). Incubation of LCM with TGF-β neutralizing antibody, prior to ESC exposure, prevented repression of BMP-2 stimulated LIF expression.

* denotes statistical significance with p<0.05