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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nature. 2014 Dec 10;517(7533):227–230. doi: 10.1038/nature14043

Extended Data Figure 2. Isothermal titration calorimetry measurement of the heat of hydrolysis of p-nitrophenylphosphate by alkaline phosphatase.

Extended Data Figure 2

Each peak corresponds to the hydrolysis of 0.053 μmol of substrate. The reaction rate slows as more substrate is added owing to the accumulation of phosphate product inhibiting the enzyme.