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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Biochem Funct. 2014 Oct 7;32(8):675–682. doi: 10.1002/cbf.3071

Figure 1. Induction of cyp24a1 mRNA expression by 25D and 1,25D identifies a functional 25-hydroxyvitamin D-1-hydroxylase in zebrafish.

Figure 1

Zebrafish larvae (day 5) were treated with increasing doses of: A) 25D (5-150 nM), B) 1,25D (0.1-10 nM) or vehicle (0.1% ethanol) for six hours, and expression of cyp24a1 mRNA determined by qRT-PCR. Data are expressed as ΔΔCt = ΔCt (cyp24a1) - ΔCt(elfa). C) Zebrafish larvae (day 5) were treated with increasing doses of itraconazole (ITRA, 0, 0.1, and 1.0 μM) one hour prior to six hour incubation with vehicle, 5 nM 25D or 0.1 nM 1,25D. Expression of cyp24a1 was then determined by qRT-PCR and expressed relative to vehicle ITRA set to 100%. Fold = 2ΔΔCt. Data are shown as n=3 replicates ± SD. * P < 0.05.