. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: Cell Biochem Funct. 2014 Oct 7;32(8):675–682. doi: 10.1002/cbf.3071

Table 1. Oligonucleotide primer sequences used for cloning.

Cloning Step	Oligo name and sequence
Middle fragment PCR	(1F) 5′-CATGTGGAAGGCCAGTTTCG-3′ (4R) 5′-TACACCTCCAGCTCAGCGAT-3′
Nested 5′ RACE PCR Reaction 1	Forward 5′ RACE outer from Ambion 5′-GCTGATGGCGATGAATGAACACTG-3′ (2R) 5′-GGTCAGCAGAGTCATCACGA-3′
Nested 5′ RACE PCR Reaction 2	Forward 5′ RACE inner from Ambion 5′-CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG-3′ (1R) 5′-CGAAACTGGCCTTCCACATG-3′
Nested 3′ RACE PCR Reaction 1	(3F) 5′-CACGATCTCCAGCACACTGT-3′ Reverse 3′ RACE outer from Ambion 5′-GCGAGCACAGAATTAATACGACT-3′
Nested 3′ RACE PCR Reaction 2	(4F) 5′-ATCGCTGAGCTGGAGGTGTA-3′ Reverse 3′ RACE inner from Ambion 5′-CGCGGATCCGAATTAATACGACTCACTATAGG-3′
Splice overlap Extension PCR 5′ RACE + Middle fragment as template	Forward 5′ RACE inner from Ambion 5′-CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG-3′ (4R) 5′-TACACCTCCAGCTCAGCGAT-3′
Splice overlap Extension PCR Middle fragment + 3′ RACE as template	(1F) 5′-CATGTGGAAGGCCAGTTTCG-3′ Reverse 3′ RACE inner from Ambion 5′-CGCGGATCCGAATTAATACGACTCACTATAGG-3′