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. 2015 Mar 17;10(3):e0120721. doi: 10.1371/journal.pone.0120721

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© 2015 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Various tissues of 18–20-week-old type 3 SMA mice were collected. (A) Total RNA was isolated and subjected to qRT-PCR to detect Tra2-β1 and ASF/SF2 mRNA levels. The result showed that among the tissues examined, the testis expressed the highest level of Tra2-β1 mRNA, but not the highest level of ASF/SF2 mRNA. (B) Proteins were extracted and subjected to Western blotting to detect Tra2-β1 and ASF/SF2 proteins. The result also showed that among the tissues examined, the testis expressed the highest level of Tra2-β1 protein, but not the highest level of ASF/SF2 protein.