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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Microb Pathog. 2015 Feb 16;80:27–38. doi: 10.1016/j.micpath.2015.02.005

Figure 5. The cytokine and nitrite production as well as the zymosan uptake of macrophages elicited by infection with WT CO92 and its Δlpp Δpla double mutant.

Figure 5

Macrophages were infected with WT CO92 and its Δlpp Δpla double mutant at MOI of 1, washed, and then subjected to gentamicin treatment. Culture supernatants were collected immediately after gentamicin treatment for analyzing cytokine production by using 6-plex Bio-Rad multiplex assay (A) or at 12 h post infection for the production of nitrite by using the Griess assay (B). For the zymosan uptake (C), the above infected macrophages were incubated with 1 × 106 (1 μm) of Texas-Red zymosan particles after the gentamicin treatment. The number of macrophages positive for beads was enumerated by flow cytometry. The dotted line represents percent of uninfected macrophages positive for zymosan uptake. Statistical analysis was performed by One-way ANOVA with Tukey’s post-hoc test, and the statistical significant difference is indicated by an asterisk with p<0.05 when compared to the uninfected control macrophages (*p<0.05). The pooled data from three independent experiments were shown and error bars indicated standard deviations.