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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jan 24;1851(5):527–536. doi: 10.1016/j.bbalip.2015.01.012

Figure 5. SR-BI in the liver, brown adipose tissue and siRNA transfected Hepa 1-6 cells.

Figure 5

(A) SR-BI, PDZK1 and NHERF1 in the liver of 2 wild type and 2 SNTA/B2 null mice. (B) SR-BI protein in the liver of 8 WT and 8 SNTA/B2−/− mice fed a HFD for 25 weeks. (C) SR-BI mRNA in the liver of 8 WT and 7 SNTA/B2−/− mice fed a HFD for 25 weeks. (D) PDZK1 protein in the liver of 7 WT and 7 SNTA/B2−/− mice fed a HFD for 25 weeks. (E) CYP7a1 mRNA in the liver of 8 WT and 7 SNTA/B2−/− mice. (F) SR-BI in brown adipose tissue of a wild type and a SNTA/B2 null mouse fed a HFD for 25 weeks. (G) SR-BI protein in brown adipose tissue of 8 WT and 8 SNTA/B2−/− mice fed a HFD for 25 weeks. (H) SR-BI, PDZK1, SNTA and SNTB2 in Hepa1-6 cells transfected with scrambled (Scr) siRNA, SNTA siRNA, SNTB2 siRNA or both. (I) PDZK1 in the liver of wild type and SNTA/B−/− mice. Original magnification of the upper panels was 20-fold and of the lower panels 60-fold. The respective IgG isotype controls are shown on the right. (J) SR-BI was immunoprecipitated in liver lysates of WT and SNTA/B2−/− mice. SR-BI, PDZK1 and SNTA were analyzed by immunoblot in the immunoprecipitates, respective control experiments and the liver lysates used.