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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Protein Expr Purif. 2015 Jan 5;108:9–12. doi: 10.1016/j.pep.2014.12.017

Figure 1.

Figure 1

SDS-PAGE and size exclusion chromatography of the expressed and purified κIO18/O8 VL. A. SDS-PAGE was performed using a 12.5% gel. Lane 1 is a whole cell extract of the E. coli cells from which the recombinant VL was purified. Lane 2 is the protein that was purified from the periplasmic fraction of the cells. The positions of MW markers which were also run on the gel are indicated. B. The elution profile obtained when the purified recombinant VL was subjected to size exclusion chromatography is shown with the positions of elution of other proteins of known molecular weight indicated.