Table 1.

Sequences of the synthetic gene used to express κI O18/O8 V_L and the protein that is encoded by the gene.

ATGATTTCCGCTACCAAAATCCGCTCATGCCTCGCGGCCTGTGTCTTGGCTGCCT TTGGAGCCACCGGAGCCCTTGCCTCGACATTCAAATGACTCAATCCCCGTCATCC CTGTCAGCGAGTGTCGGTGATCGCGTCACGATCACGTGCCAGGCGTCTCAAGACA TTAGCAACTACCTGAATTGGTACCAGCAGAAACCAGGTAAGGCCCCGAAACTCTTG ATCTACGACGCGTCCAATTTGGAAACAGGCGTGCCGAGTCGCTTTAGCGGTAGCG GAAGCGGCACCGATTTCACCTTCACCATCAGTTCCCTTCAGCCGGAAGACATCGC CACCTACTATTGTCAACAGTATGACAATCTGCCATATACGTTTGGCCAGGGCACCA AACTGGAAATCAAGCACCATCATCATCATCATTAG

M I S A T K I R S C L A A C V L A A F G A T G A L A D I Q M T Q S P S S L S A S V G D R V T I T C Q A S Q D I S N Y L N W Y Q Q K P G K A P K L L I Y D A S N L E T G V P S R F S G S G S G T D F T F T I S S L Q P E D I A T Y Y C Q Q Y D N L P Y T F G Q G T K L E I K H H H H H H

(Top) The DNA sequence of the gene encoding κIO18/O8 V_L with the N-terminal mauC signal sequence and the sequence encoding the C-terminal hexahistidine tag underlined.

(Bottom) The amino acid sequence encoded from this gene is shown. The N-terminal signal sequence which is cleaved during expression and hexahisidine tag that is used for affinity purification are underlined.