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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Stem Cell Res. 2015 Jan 6;14(2):144–154. doi: 10.1016/j.scr.2014.12.007

Figure 3. H2A.Z asymmetry in prophase nuclei detected by phosphorylated histone H3 in cultures of wild-type, pre-crises MEFs.

Figure 3

DAPI, nuclear DNA fluorescence. H2A.Z, ISIF with antibodies specific for H2A.Z. pH3, ISIF with antibodies specific for phosphorylated histone H3. ISIF was performed simultaneously for pH3 and H2A.Z. A-C, independent fluorescence images for the indicated markers. D-F, merged combinations of A-C images as denoted. Yellow solid arrows, example of a prophase nucleus with H2A.Z asymmetry. White dotted arrows, example of a prophase nucleus with symmetric H2A.Z. si, example of interphase nucleus (i.e., pH3-negative) with symmetric H2A.Z localization. zn, H2A.Z-negative interphase nucleus. G and H, 10X magnification of nuclear images in the inset boxes of D and E, respectively. Scale bar = 25 microns.