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. 2015 Mar 10;15:13. doi: 10.1186/s12861-015-0060-2

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© O'Doherty et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic representation of samples and imprinted genes analysed. (A) Location of imprinted gene CpG islands (green box labelled GGI) and CpG dinucleotides covered by pyrosequencing analysis. Genes investigated were (clockwise from top left); SNRPN, MEST, PLAGL1, H19, PEG10 and IGF2R. (B) in vivo embryos from non-stimulated, artificially inseminated heifers were isolated 7, 14, 17 and 25 Days post insemination and used for imprinted gene DNA methylation analysis. ICM = inner cell mass; TE = trophectoderm adjacent to embryonic disc; TP = trophectoderm peripheral; ExEmb = extraembryonic and Emb = embryonic.