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. 2014 Dec 4;15(1):1064. doi: 10.1186/1471-2164-15-1064

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© Sandh et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Visualization of the heterocyst envelope and the cell-type specific relative abundance levels of proteins encoded by the hgl gene cluster. Staining of (A) N₂-fixing filaments and (B) the isolated heterocyst fraction from the same diazotrophic culture with (5%) alcian blue. A high degree of heterocyst purity was evident in the isolated fraction. (A) Size bar =10 μm, (B) Size bar =5 μm. (C) All proteins encoded within the hgl gene cluster (Additional file 1) showed higher relative protein abundance in heterocysts. The cell-type specific protein ratios are given as Log₂ values (heterocysts/N₂-fixing filaments) from samples taken 24 hours after the removal of combined nitrogen. *Indicates that the protein was detected in only one of the two biological replicas.